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Why is RNA universal co-extraction kit particularly suitable for RNA extraction from complex agricultural and animal husbandry samples?

Publish Time: 2025-07-22
In agricultural and animal husbandry research, disease monitoring and food safety testing, RNA extraction is a key step in pathogen detection, gene expression analysis, virus screening and other research. However, agricultural and animal husbandry samples are of various types and complex components, such as plant tissues, animal tissues, blood, feces, soil, dairy products, etc. These samples often contain a large amount of polysaccharides, polyphenols, proteins, lipids and PCR inhibitors, which poses a great challenge to RNA extraction. RNA universal co-extraction kit is designed to meet this practical demand. It has core advantages such as high efficiency, high purity and broad spectrum compatibility, making it an ideal tool for RNA extraction from complex agricultural and animal husbandry samples.

1. Designed specifically for complex agricultural and animal husbandry samples, with a wide range of applications

One of the biggest features of RNA universal co-extraction kit is its wide sample adaptability. It is not only suitable for common animal tissues (such as liver, lungs, spleen, blood, etc.) and plant tissues (such as leaves, fruits, seeds, etc.), but can also effectively handle difficult samples such as feces, soil, milk samples, and semen. These samples usually contain a large number of impurities that inhibit RNA extraction and downstream detection, and traditional extraction methods often make it difficult to obtain high-quality RNA. The kit can effectively lyse cell walls, cell membranes and virus particles through an optimized lysis buffer and purification column system to release RNA, and remove interfering substances such as polysaccharides, proteins, and lipids through efficient purification technology to ensure the integrity and availability of RNA. This "co-extraction" capability allows one kit to meet the RNA extraction needs of multiple sample types, greatly improving experimental efficiency and operational convenience.

2. Efficient extraction capability to ensure accurate detection of low-concentration samples

In agricultural and animal husbandry testing, many samples have low RNA content, especially viral RNA and microbial RNA in environmental samples, which places extremely high demands on the sensitivity of the extraction method. RNA universal co-extraction kit uses high-sensitivity RNA enrichment technology to effectively capture trace RNA in low-concentration samples to ensure its detectability in subsequent testing. In addition, the lysis solution and binding solution in the kit are specially formulated and optimized to enhance the binding efficiency of RNA and purification columns, reduce the loss of RNA during washing, and thus significantly improve the recovery rate. Even for samples that are extremely challenging to extract RNA (such as viral RNA in feces or bacterial RNA in soil), sufficient RNA can be obtained for subsequent molecular tests such as qRT-PCR, RT-PCR, and NGS library construction to ensure the accuracy and reliability of the test results.

3. High-purity extraction to ensure compatibility with a variety of PCR reagents

The purity of RNA directly affects the results of downstream molecular experiments. Traditional extraction methods are prone to residual impurities such as proteins, polysaccharides, and ethanol, which interfere with the reverse transcription reaction or PCR amplification efficiency and even lead to false negative results. RNA universal co-extraction kit uses a multi-step purification mechanism, including efficient RNA binding columns, specific eluents, and detergent systems to effectively remove interfering substances and obtain high-purity, inhibitor-free RNA samples. The extracted RNA OD260/OD280 ratio is stable between 1.8 and 2.1, meeting the standards of high-quality RNA and suitable for a variety of downstream applications, including fluorescence quantitative PCR (qRT-PCR), conventional reverse transcription PCR (RT-PCR), microarray analysis, gene expression research, viral load detection, etc. At the same time, its extraction products have good compatibility with mainstream PCR kits on the market (such as TaqMan, SYBR Green, etc.), and can be directly used for detection without additional purification, which greatly improves the experimental efficiency.

4. Easy operation, good repeatability, suitable for large-scale sample processing

The design of RNA universal co-extraction kit fully considers the convenience and standardization requirements of laboratory operations. Its operation process is simple and clear, and only a few steps of lysis, centrifugation, washing, and elution are required to complete RNA extraction. The whole process can be completed within 30 minutes. The matching purification column has a stable structure and high batch reproducibility, ensuring the consistency and repeatability of experimental results. In addition, the kit supports 96-well plate high-throughput operation, which is suitable for automated nucleic acid extraction platforms to meet the large-scale sample processing needs of agricultural and animal husbandry disease monitoring, food safety testing, scientific research projects, etc. In application scenarios such as animal disease prevention and control centers, agricultural testing institutions, and animal husbandry and veterinary laboratories, it can significantly improve detection efficiency and shorten response time.

5. Environmentally friendly and safe, in line with the requirements of modern laboratories

The RNA universal co-extraction kit focuses on environmental protection and safety in its formula design, avoids the use of toxic reagents such as phenol and chloroform, and adopts non-toxic or low-toxic alternative reagents, which not only ensures the safety of operators, but also reduces the pressure of laboratory waste liquid treatment. At the same time, the reagent has good stability, long shelf life, is easy to transport and use on site, and is particularly suitable for special scenarios such as field sampling and mobile testing.

With its wide sample applicability, efficient extraction capacity, high-purity RNA output, good downstream compatibility and easy operation, the RNA universal co-extraction kit has become an ideal choice for RNA extraction from complex samples in agriculture and animal husbandry. It not only meets the needs of scientific researchers for high-quality RNA, but also provides strong technical support for practical applications such as agricultural and animal husbandry disease monitoring, food safety testing, and pathogen screening.
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